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A. V. Ivanov, M. S. Vakshtein, P. N. Nesterenko

Formation of Decreasing pH Gradient within Cation-Exchange Carboxylic Columns


The possibility of decreasing pH gradient formation by chromatofocusing technique within columns, packed with carboxylic cation-exchangers, was demonstrated. The Polybuffer-96 solution (1:20) was used as eluent forming pH gradient. The linearity of pH gradient profile is achieved by variation of ionic strength of start buffer solution and eluent.
Moscow University Chemistry Bulletin.
2002, Vol. 43, No. 3, P. 180

Copyright (C) Chemistry Dept., Moscow State University, 2002
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