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Filatova L. Y., Donovan D. M., Novozhilov I. A., Chubar T. A., Balabushevich N. G., Pugachev V. G., Klyachko N. L.
Influence
of block-copolymers of different nature on staphylolytic lysk enzyme secondary
structure and stability
Abstract
The branched block-copolymer of polyethyleneimine and polyethylene
glycol PEI-PEG and the linear block copolymer of polylysine and polyethylene
glycol PLL-PEG forms complexes with the staphylolytic LysK enzyme. Complexing
between LysK and cationic block-copolymers causes 1.5–2 times increase of the
enzyme lytic activity. Block-copolymers stabilize LysK at the temperatures of
storage (4 and 22 °C), and the half-inactivation time increases by 2–20 times.
Stabilizing effects of block copolymers depend on temperature, NaCl
concentration, polymer/enzyme ratio. Linear PLL-PEG provides an increase in
LysK stability suitable for biomedical materials (LysK retains 100% activity
after 4 months at 4 °C).
Key words: Staphylococcus
aureus, lytic enzymes of bacteriophages, polymer, structure,
stability.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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