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G. V. Presnova, D. E. Presnov, V. A. Krupenin, M. M. Ulyashova, A. M. Egorov, M. Yu. Rubtsova
Multianalysis
of thyroid cancer markers on porous membrane surface and semiconductor supports
using gold nanoparticles as label
Abstract
We present a method for simultaneous determination of
thyroglobulin and thyroid-stimulating hormone on microchips using gold
nanoparticles for antibodies labeling. Nitrocellulose and silicon were used as
supports for microchips. The determination principle is based on the
sandwich-immunoassay using a pair of antibodies that simultaneously bind to the
analyte. Specific monoclonal antibodies providing better sensitivity
and specificity for determination of hormones, as well as a better
signal-to-background ratio where selected. The analytical signal was the
intensity of microchip spots staining on the membrane carrier and the density
of nanoparticles on silicon. The detection limit for thyroglobulin and
thyroid-stimulating hormone was found of 0.25 ng/ml and 0.016 μIU/ml
on a membrane support, and of 0.1 ng/ml and 0.013 μIU/ml
on silicon, respectively. The method can be further used for developing
nanosensor devices for express
diagnostics of thyroid pathologies.
Key words: microchips, sandwich immunoassay, gold nanoparticles,
oncomarkers, thyroid hormones.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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