M. A. Rubtsov, A. A. Maslakova, D. M. Potashnikova, V. P. Veiko, M. S. Syrkina
Tetrameric RGD induces clustering of
integrin αvβ3on the melanoma cell surface and decrease of cell viability
The recombinant hybrid protein SR15
composed of streptavidin fused with RGD-bearing peptide was obtained. Itís
selective binding to human melanoma cells (MeWo) was demonstrated. Composition
of the RGD-binding integrins on the surface of MeWo cells was identified. It
was established that the recombinant protein SR15 binds to integrin αvβ3
on the surface of human melanoma cells. It was also shown that such binding
induces clustering of αvβ3 receptors on the surface of MeWo cells
with following internalization of the recombinant
protein and results in twofold decrease in cell viability.
Key words: RGD,
streptavidin, human melanoma, MeWo, integrin, αvβ3, receptor clustering.
Copyright (C) Chemistry Dept., Moscow State University, 2002