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M. M. Ulyashova, G. V. Presnova, Yu. I. Pobolelova, A. A. Filippova, A. M. Egorov, M. Yu. Rubtsova*

Screening of bacterial genes responsible for resistance to beta-lactam antibiotics using microarrays with enzymatic detection


The method of hybridization analysis on microarrays with enzymatic detection based on horseradish peroxidase was applied for screening the pathogens of nosocomially-acquired and community-acquiredinfections for the presence of genes of beta-lactamases, causing resistance to beta-lactam antibiotics. The advantages of this method for the rapid identification of genes were shown. Similarities and differences in the distribution of beta-lactamase genes for nosocomially-acquired and community-acquired infections were revealed. The most common ESBL type was CTX-M type of beta-lactamases. The high prevalence of beta-lactamase TEM-1 was detected. Beta-lactamase subgroup CTX-M-1 alone or in combination with genes of beta-lactamases TEM-1 and SHV-1 was most frequently identified for community-acquired infections. No cases of simultaneous detection of multiple ESBLs for community-acquired pathogens have been detected. Much more varied combinations of beta-lactamases were identified for nosocomially-acquired infections: a combination of ESBL and other beta-lactamases - in 62% of strains, the simultaneous presence of two different types of ESBLs in 18% of strains.
Key words: beta-lactamases, microarrays, hybridization, colorimetric detection, horseradish peroxidase.
Moscow University Chemistry Bulletin.
2016, Vol. 57, No. 4, P. 245

Copyright (C) Chemistry Dept., Moscow State University, 2002
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