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A. V. Shnitko, M. G. Chernysheva, S. A. Smirnov, P. A. Levashov, G. A. Badun
Pluronics and Brij-35 reduce the
bacteriolytic activity of lysozyme
Abstract
The influence of non-ionic surfactants on
the bacteriolytic activity of lysozyme was studied on the model cells
Micrococcus luteus. Pluronics P123, L121 and F127 as well as Brij-35 were used.
The formation of complexes of these compounds and lysozyme occurs via formation
of hydrogen bonds between ethylene oxide fragments of the surfactant and amino
acids residues of lysozyme surface. The bacteriolytic activity of lysozyme was
reduced in line F127 < P123 < L121 ≈ Brij-35 probably because of
steric hindrances of the protein interaction with cells substrate.
Key words: lysozyme, pluronics, non-ioinic
surfactants, bacteriolytic activity.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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