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T. A. Bogush, S. A. Kaliuzhny, A. A. Basharina, A. N. Grishanina, Yu. B. Dyakova, E. A. Bogush, V. Yu. Kirsanov, M. M. Davydov
Immunofluorescence analysis of vimentin
expression in epithelium cells
Abstract
A new method of dual immunofluorescence
analysis by flow cytometry was developed to evaluate the quantitative expression
of mesenchymal marker vimentin in epithelium cells of solid tumor, which in its
turn is a “mixture” of different histogenesis cells. The method has a number of
evident advantages as compared to clinically used immunohistochemistry
technique for marker investigation: intermediate precision of results, gentle
pre-analytical preparation, lack of subjectivity in results interpretation and
integral evaluation of markers expression in a large number of cells from a
large volume tumor tissue sample (≥5k cells out of ≥2 cm in sample
diameter).
Key words: immunofluorescence analysis,
flow cytometry, vimentin, cytokeratins, epithelial-to-mesenchymal transition.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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