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D. V. Vokhmyanina, E. E. Karyakina, E. A. Andreev, A. A. Karyakin
Thin-film Prussian
Blue based multibiosensor for glucose and lactate simultaneous determination
Abstract
Multibiosensor
for simultaneous determination of glucose and lactate has been developed by
combination of the advantageous transducer for hydrogen peroxide reduction
(Prussian Blue) and protocol of enzyme immobilization from water-organic
mixtures with a high content of organic solvent. The analytical characteristics
of the resulted biosensor were: the sensitivity of > 80 mA∙M–1∙cm–2 for lactate
detection and > 20 mA∙M–1∙cm–2 for glucose detection, linear dynamic range
of 1–500 µM for lactate and 5–1000 µM for glucose as well as high operational stability (100 measurements
without calibration). The applicability of developed multibiosensor for routine
blood analysis has been shown. Electroanalytical system based on multibiosensor
reveals improved analytical performances compared to commercial analyzers.
Key words:
Prussian Blue, multibiosensor, flow injection analysis, thin-layer cell,
glucose, lactate.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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