ChemNet
 
Previous article Contents  

A. A. Pometun, N. S. Voinova, E. V. Pometun, S. S. Savin, V. I. Tishkov

Impact of medium pH and ion strength on thermal stability of plant formate dehydrogenases

Abstract

Impact of medium pH and ion strength on thermal stability of formate dehydrogenases (EC 1.2.1.2, FDH) from model plant Arabidopsis thaliana (AthFDH) and soya Glycine max (SoyFDH) have been studied. Dependence of residual activity on time followed kinetics of reaction of the first order at all experimental conditions.. Dependences of first order thermal inactivation constants showed bell-shape curve. It was found that pH increase resulted in growth of inactivation rate constant for SoyFDH and decrease of one for AthFDH. Activation parameters of thermal inactivation process, ΔH and ΔS, have been calculated from experimental data at different medium pH values and phosphate buffer concentrations.
Key words: formate dehydrogenase, thermal stability, impact of medium parameters, activation parameters of thermal inactivation process.
Moscow University Chemistry Bulletin.
2018, Vol. 59, No. 4, P. 313
   

Copyright (C) Chemistry Dept., Moscow State University, 2002
   Overview
   Editorial board
   Tables of Contents
   Subscription

The site is supported by Russian Foundation for Basic Research
  The using of published on this page materials is not allowed without special permission
Copyright (C) Chemisty Department of Moscow State University
Web-Editor: B.I.Pokrovskii
Web-design: Copyright (C) MIG and VVM
webmaster@www.chem.msu.su