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V. A. Kryachkov, V. N. Tashlitsky, A. V. Bacheva

Design and characteristics on novel proteasome substrates containing oligoQ sequence

Abstract

Fluorogenic polyglutamyl-containing peptides with 5 or 10 Gln residues and FRET-pair EDANS (fluorophore) and Dabcyl (quencher) were characterized by spectral and mass-spectrometry techniques. The possibility for 20S proteasome to hydrolyze these modified peptides was examined. Hydrolysis kinetics was studied and catalytic efficiency for the substrates was determined. It was shown that presence of glycine dramatically decreased the solubility of the substrate and also diminished the efficiency of the hydrolysis.
Key words: proteasome, fluorogenic substrates, proteasome activity, polyglutamine diseases.
Moscow University Chemistry Bulletin.
2018, Vol. 59, No. 4, P. 275
   

Copyright (C) Chemistry Dept., Moscow State University, 2002
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