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K. V. Serebrennikova, J. V. Samsonova, A. P. Osipov

Improved sensitivity of lateral flow immunoassay using the biotin-streptavidin system


A new approach for improving the sensitivity of lateral flow immunoassay based on the use of a complex conjugate is proposed. The complex conjugate is an agglomerate of gold nanoparticles formed by binding biotinylated antibodies labeled with gold nanoparticles to streptavidin. The amplification of the analytical signal occurs due to the accumulation of agglomerates of gold nanoparticles in the test zone of the strip. The proposed test system is implemented for lateral flow immunoassay of model protein, procalcitonin, and allows one-step analysis without additional reagents or manipulations. The range of procalcitonin detection was from 0.5 to 100 ng/ml with a limit of detection at 0.25 ng/ml, which is 4 times better the sensitivity of the conventional lateral flow immunoassay using spherical gold nanoparticles as a label.
Key words: lateral flow immunoassay; gold nanoparticles; biotin; streptavidin; procalcitonin.
Moscow University Chemistry Bulletin.
2018, Vol. 59, No. 3, P. 230

Copyright (C) Chemistry Dept., Moscow State University, 2002
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