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K. V. Serebrennikova, J. V. Samsonova, A. P. Osipov
Improved
sensitivity of lateral flow immunoassay using the biotin-streptavidin system
Abstract
A new approach
for improving the sensitivity of lateral flow immunoassay based on the use of a
complex conjugate is proposed. The complex conjugate is an agglomerate of gold
nanoparticles formed by binding biotinylated antibodies labeled with gold
nanoparticles to streptavidin. The amplification of the analytical signal
occurs due to the accumulation of agglomerates of gold nanoparticles in the
test zone of the strip. The proposed test system is implemented for lateral
flow immunoassay of model protein, procalcitonin, and allows one-step analysis
without additional reagents or manipulations. The range of procalcitonin
detection was from 0.5 to 100 ng/ml with a limit of detection at 0.25 ng/ml,
which is 4 times better the sensitivity of the conventional lateral flow
immunoassay using spherical gold nanoparticles as a label.
Key words:
lateral flow immunoassay; gold nanoparticles; biotin; streptavidin;
procalcitonin.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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