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A. A. Pometun, S. Yu. Kleymenov, S. A. Zarubina, I. S. Kargov, P. D. Parshin, E. G. Sadykhov, S. S. Savin, V. I. Tishkov
Comparison of
thermal stability of new formate dehydrogenases with differential scanning
calorimetry
Abstract
Systematic study
of formate dehydrogenases (FDH) from different sources are carried out in our
laboratory. Recently, new genes encoded FDH from pathogenic bacterium Staphylococcus
aureus (SauFDH), methylotrophic thermotolerant yeast Ogataea
parapolymorpha (OpaFDH), yeast Saccharomyces cerevisiae (SceFDH) and
moss Physcomitrella patens (PpaFDH) were cloned and expressed in our
laboratory. The comparative analysis of thermal stability for new recombinant
formate dehydrogenases was made with differential scanning calorimetry. It was
shown that two new enzymes – SauFDH and OpaFDH were comparable in stability to
FDH from bacterium Pseudomonas sp 101. SceFDH showed the lowest
thermalstability compare to all described formate dehydrogenases.
Key words:
thermal stability, formate dehydrogenase, differential scanning calorimetry.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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