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M. K. Gubaidullina, A. E. Urusov, A. V. Zherdev, C. Xu, B. B. Dzantiev
Immunochromatographic
test systems using the conjugate anti-species antibodies – colloid gold:
features and benefits on the example of determination of ochratoxin A
Abstract
In the present
work, new format of immunochromatographic analysis with indirect labeling was
compared with a traditional analysis for the case of ochratoxin A (OTA). In the
proposed scheme specific antibodies are included in the dilution buffer, while
the anti-species antibody conjugate with the marker is applied to the test
strip. The assay is realized for the detection of OTA in maize extracts. The
indirect labeling reduces the detection limit of OTA by two orders of magnitude
(up to 0.12 ng/mL). The reasons for this gain are considered. The approach can
be applied to various low molecular weight compounds.
Key words:
immunoassay, test-strips, colloidal gold, antibody – nanoparticle complexes,
mycotoxins.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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