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K. V. Serebrennikova, J. V. Samsonova, A. P. Osipov
Gradient lateral
flow immunoassay of human chorionic gonadotropin
Abstract
A new format of visual semi-quantitative express
method, which is based on the principle of gradient lateral flow immunoassay
with gold nanoparticles as a label was proposed. Several test lines with
increasing concentration of specific antybodies immobilized on the membrane
were formed in the analytic zone of the test strip. Semi-quantitative analysis
of antigen was carried out visually by counting colored lines in analytic zone.
As a model antigen the human chorionic gonadotropin was chosen and the possibility
of semi-quantitative determination of analyte without any device in the range
from 50 to 10000 mU/mL during 5–10 minutes in five concentration ranges: less
than 100 mU/mL; 100–300; 300–500; 500–800 and more than 800 mU/mL was shown.
The developed semi-quantitative express method allows to determine the
concentration range of human chorionic gonadotropin in urine by number of
colored lines for diagnosis of pregnancy and its course.
Key words:
gradient lateral flow immunoassay; human chorionic gonadotropin; gold
nanoparticles.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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