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T. A. Bogush, I. A. Mamichev. S. A. Kaliuzhny, B. E. Polotskii, V. T. Zarkua, A. S. Tjulandina, E. A. Bogush, M. M. Davydov
Comparative
analysis of data obtained by immunofluorescence estimation of ERCC1 expression
using different clones of monoclonal antibodies 8F1 and FL297
Abstract
In the present research, we compared two datasets obtained from the
quantitative estimation of ERCC1 protein using two monoclonal antibodies – 8F1
and FL29. Quantitative evaluation of immunofluorescence parameters was performed
by flow cytometry on ovarian cancer biopsy specimens (n = 53). Agreement
between two different assays was estimated by correlation analyses and
Bland-Altman plots. It was shown that, despite of large spread of differences
between two measurements obtained by 8F1 and FL297, both clones may be used for
estimation of ERCC1 expression level (i.e. number of positively stained cells)
in tumor.
Key words: immunofluorescence analysis, flow cytometry, serous ovarian
cancer, ERCC1.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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