|
E. N. Shapovalova, I. A. Fedorova, A. A. Priporova, I. A. Ananieva, O. A. Shpigun
Determination
of the enantiomeric purity of albuterol on the macrocyclic glycopeptides bonded
phases
Abstract
In
this research, the separation of albuterol enantiomers on silica gel modified
by macrocyclic glycopeptides (eremomycin – column Nautilus-E, BioChemMac,
Russia and teicoplanin aglycone – column Chirobiotic TAG, Astec, USA was
investigated. Enantiomers of albuterol were separated on Nautilus-E and
Chirobiotic TAG columns in polar-organic mode chromatography. The selectivity
was higher on Chirobiotic TAG column (Rs = 1.7). The maximum
resolution of enantiomers peaks (1.7) was obtained when mobile phase was
methanol:acetonitrile:triethylamine:CH3COOH (90:10:0.5:0.5). The
detection limit of the compound, calculated by the signal / background ratio =
3:1 was 0.2 µg/ml, it correspond 0.10% S-enantiomer of total composition of
drug. The content of S-albuterol is permitted at no greater than 0.2%. The
determination of enantiomeric purity of the pharmaceutical substances was
carried out.
Key words: macrocyclic glycopeptides, chiral selector, enantiomers, enantiomeric
purity of the pharmaceutical substances.
Copyright (C) Chemistry Dept., Moscow State University, 2002
|
|
