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E. V. Semina, K. A. Rubina, V. Yu. Sysoeva, V. V. Stepanova, V. A. Tkachuk
3D-model of biomatrix
as a method of studying blood vessels and nerves growth in tissue engineering
structures
Abstract
The method involves
isolating and culturing ex vivo cultures of mouse dorsal root ganglia and
abdominal aorta in 3-dimensional gel (Matrigel). Unlike other explant cultures,
this technique allows us to study the physiological and biochemical processes
in the tissue explants of dorsal root ganglia and abdominal aorta in
three-dimensional space but not in a two-dimensional adhesive culture.
Introduction to the Matrigel testing substances allows analyzing their effects
on the dynamics of the explants for 21 days. The developed method can be
applied in modern cardiology and neurobiological research and explores how new
therapeutic agents can accelerate the regeneration of blood vessels and nerves.
Key words:
3-dimensional explant culture, dorsal root ganglia, abdominal aorta,
regenerative medicine.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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