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E. G. Vlakh, G. A. Platonova, T. B. Tennikova
Preparation and study of flow-through
bioreactors based on macroporous monoliths
Abstract
The modification of surface of macroporous
monolithic disks with hydrolytic enzymes, e.g. α-chymotrypsin
and ribonuclease A, has been described. The influence of the enzyme
immobilization approach, amount of the aldehyde groups in the polymer spacer as
well as flow rate of substrate solution recirculation on the efficiency of
heterogeneous biocatalysis was explored using as examples the reactions of
hydrolysis both low and macromolecular substrates.
Key words: immobilized enzymes,
macroporous monolithic carriers, flow-through biocatalysis, hydrolases.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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