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G. V. Presnova, D. E. Presnov, V. G. Grigorenko, A. M. Egorov, M. Yu. Rubtsova
Orientedimmobilization of antibodiesand their fragmentson the modifiedsiliconto createnanosensors
Abstract
Diferent methods for covalent
immobilization of specific antibodies and their fragments on silicon surface
with the subsequent formation of immune complexes, consisting of immobilized
monoclonal antibody, an antigen molecule and a second molecule of the
monoclonal antibody labeled with gold nanoparticles, were studied. A new method forthe chemical modification ofsilicon with 3-aminopropyltrimetoxysilane (APTMS) and the bifunctional reagent 1,
4-phenylene diisothiocyanate (PDITC) was developed. It provides
uniform distributionand availability ofantigen-bindingcentersfor the formation ofimmune complexes.
Gold nanoparticles were used as labels for the immune complexes, and they were
introduced through the interaction of biotin with streptavidin. Scanning
electron microscopy (SEM) was applied for the registration of immune complexes
on the surface. High resolution of the method allowed the registration of the
immune complexes by the presence of gold nanoparticles and count their number.
The methoddeveloped is promisingfor the formation of biospecificlayersonbiosensors based onsilicon nanowires.
Key words: specific
antibodies, antigen-antibody interaction,
gold nanoparticles, scanning electron
microscopy.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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