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O. A. Kost, M. N. Petrov, I. A. Naperova, V. A. Tikhomirova, O. V. Kryukova, I. V. Gachok, N. I. Bulaeva, E. Z. Golukhova, S. M. Danilov

Conformational "fingerprinting" of angiotensin-converting enzyme in the blood in health and disease

Abstract

The pattern of binding of the set of 16 monoclonal antibodies to the different epitops on the surface of two domains of angiotensin-converting enzyme – “conformational fingerprinting” – allows to reveal in the blood the presence of the enzyme, which is not produced by lung endothelial cells, but by other cells, e.g. by Gaucher’s cells and sarcoid granuloma cells. We showed the existence of conformationally changed angiotensin-converting enzyme in the blood of patients with uremia, this enzyme being characterized by the enhanced activity towards angiotensin I and decreased ability to be inhibited by specific inhibitors. The perspectives of the discovering of conformationally changed enzyme in the blood at atrial fibrillation are also discussed.
Key words: angiotensin-converting enzyme, monoclonal antibodies, conformation, tissue specificity.
Moscow University Chemistry Bulletin.
2015, Vol. 56, No. 6, P. 399
   

Copyright (C) Chemistry Dept., Moscow State University, 2002
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