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E. M. Basova, V. M. Ivanov, O. C. Apendeeva

Potential of Fluorat-02 Panorama spectrofluorImeter for the analysis of fluorescent dyes mixtures

Abstract

The fluorescent dyes fluoresceine disodium, eosin Y, rhodamine B, rhodamine 6G and amino-G acid were chosen for the investigation. Their absorption, excitation and fluorescence spectra in aqueous media at different pH values were recorded on a Fluorat-02-Panorama spectrofluorimeter and the maxima of excitation and emission for individual compound were established. The excitation and emission maxima of fluorescein disodium, eosin Y. rhodamine B, rhodamine 6G and AGA were 490/514, 516/544, 529/555, 545/576 and 300/448 nm respectively under optimal pH 9,18 (borate buffer). The optimal conditions of simultaneous determination up to 5 fluorescent dyes were established. The fluorescent spectra of dyes studied overlapped. The determination of 5 dyes may be performed from fluorescence spectra at excitation wavelength 300 nm with heart cutting of wavelength range 480-580 nm. The determination of 4 dyes (except AGA) may be performed from fluorescence spectra at excitation wavelength 490 nm with heart cutting of wavelength range 500-500 nm or from synchronous fluorescence spectra with 25 nm interval between excitation and emission wavelength in the range 300-680 nm. The dye concentrations were calculated by Panorama pro. Version 2.1.0 software. The method was applied to analyze different model mixtures containing 2-5 dyes with satisfactory results.
Key words: fluorescent dyes, fluoresceine disodium, eosin Y, rhodamine B, rhodamine 6G , amino-G acid, synchronous fluorescence spectra, simultaneous determination.
Moscow University Chemistry Bulletin.
2014, Vol. 55, No. 5, P. 281
   

Copyright (C) Chemistry Dept., Moscow State University, 2002
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