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I. V. Lyagin, E. N. Efremenko, A. V. Kabanov
Catalytic characteristics of
enzyme-polyelectrolyte complexes based on hexahistidine-containing
organophosphorus hydrolase
Abstract
A number of covalent and non-covalent
polyelectrolyte complexes based on enzyme such as hexahistidine-containing
organophosphorus hydrolase were developed in the work. Polyanions and
polycations were used in the form of block-copolymers with poly(ethylene
glycol). To obtain covalent complexes of the enzyme, different coupling agents
(glutaric aldehyde, N-ethyl-N′-(3-dimethylaminopropyl)carbodiimide
hydrochloride (EDC) and N-hydroxysulfosuccinimide sodium salt) were tried. The
best catalytic efficiency of action of covalently bound complex
((2.8±0.3)×108 M–1s–1) was obtained in
the case of samples with EDC. The modification of the protein surface was
undertaken as an approach to its further biomedical application.
Key words: hexahistidine-containing
organophosphorus hydrolase, polyelectrolyte, enzyme-polyelectrolyte complex.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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