|
D. A. Myasnikova, A. E. Poliakov, O. E. Vashkinskaya, S. V. Muginova, T. N. Shekhovtsova
The
influence of hydrophilic ionic liquid nature on catalytic activity of
horseradish and soybean peroxidases
Abstract
The
kinetics of quaiacol oxidation with hydrogen peroxide
and tert-butylhydroperoxide catalyzed by
horseradish and soybean peroxidases in the presence
of 1-butyl-3-methylimidazolium acetate, tetrafluoroborates
of 1-butyl-3-methylimidazolium and N-butyl-4-methylpyridinium has been
studied and compared. The noncompetitive inhibition of
acetate ionic liquid on both peroxidases was found;
the Michaelis and inhibition constants for reactions
were calculated. The reasons for different degree of inhibition of catalytic
activities of plant peroxidases by ionic liquids are
discussed. The advantages of ionic liquids in comparison with polar molecular
organic solvents are shown.
Key words:
hydrophilic ionic liquids, horseradish and soybean peroxidases,
catalytic activity, kinetics.
Copyright (C) Chemistry Dept., Moscow State University, 2002
|
|
