Previous article Next article Contents  

V. I. Beketov, R. D. Voronina, N. B. Zorov

Fluorimetric determination of amino acids and photochemical stability of the products of their reaction with orto-phthalic aldehyde under irradiation of a high-power pulsed laser.


The possibility of high-sensitive determination of amino acids was investigated with the use of fluorescence of their derivatives obtained by reaction of aqueous solution of ortho-phthalic dialdehyde and nucleophilic additives: potassium cyanide, sodium sulfite and N-acetyl-L-cysteine. With glycine as an example, it was shown that chemical stability of yielded highly fluorescent compounds (substituted isoindoles) and their photochemical stability under irradiation of a powerful pulsed laser is substantially higher than the ones of widely used reagent for determination of amino acids - the basic aqueous solution of ortho-phthalic dialdehyde and 2-mercaptoethanol. The analytical characteristics of the newly developed reagents are the same or better than of the standard one.
Key words: amino acids, fluorimetric determination, substituted isoindoles, pulsed laser excitation, photochemical stability.
Moscow University Chemistry Bulletin.
2012, Vol. 53, No. 4, P. 228

Copyright (C) Chemistry Dept., Moscow State University, 2002
   Editorial board
   Tables of Contents

The site is supported by Russian Foundation for Basic Research
  The using of published on this page materials is not allowed without special permission
Copyright (C) Chemisty Department of Moscow State University
Web-Editor: B.I.Pokrovskii
Web-design: Copyright (C) MIG and VVM