V. I. Beketov, R. D. Voronina, N. B. Zorov
Fluorimetric determination of amino acids and photochemical
stability of the products of their reaction with orto-phthalic
aldehyde under irradiation of a high-power pulsed laser.
possibility of high-sensitive determination of amino acids was investigated
with the use of fluorescence of their derivatives obtained by reaction of
aqueous solution of ortho-phthalic dialdehyde and nucleophilic additives:
potassium cyanide, sodium sulfite and N-acetyl-L-cysteine. With glycine as an
example, it was shown that chemical stability of yielded highly fluorescent
compounds (substituted isoindoles) and their photochemical stability under
irradiation of a powerful pulsed laser is substantially higher than the ones of
widely used reagent for determination of amino acids - the basic aqueous
solution of ortho-phthalic dialdehyde and 2-mercaptoethanol. The analytical
characteristics of the newly developed reagents are the same or better than of
the standard one.
acids, fluorimetric determination, substituted isoindoles, pulsed laser
excitation, photochemical stability.
Copyright (C) Chemistry Dept., Moscow State University, 2002