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A. V. Alexeev, E. V. Proskurnina, Yu. A. Vladimirov
Determinationof antioxidantsbysensitizedchemiluminescence
using 2,2¢-azo-bis(2-amidinopropane)
Abstract
For the determination of antioxidants, an analytical procedure was
proposed based on chemiluminescence. The analytical system consists of a
free-radicals generator 2,2¢-azo-bis(2-amidinopropane)
and luminol as a chemiluminescence sensitizer in phosphate buffer solution (pH
7,4). The analytical conditions were optimized. The analytical procedure
provides detection limits as low as (μM) for: trolox – 0,05; ascorbic acid
– 0,20; quercetin – 0,03; dihydroquercetin – 0,03. The procedure was used for
determination of antioxidants in food.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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