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A. A. Bendryshev, E. B. Pashkova, A. V. Pirogov, O. A. Shpigun

Determination of water soluble vitamins in vitamin premixes, biologically active dietary supplements and pharmaceutical preparation by HPLC with gradient elution


Influence of the pH of the mobile phase and temperature on the chromatographic separation of 14 vitamins and vitameric forms on four different stationary phases was investigated. The chromatographic parameters for simultaneous determination of 12 vitamins and vitameric forms were suggested. The best separation was achieved under the following conditions: gradient elution mode, pH of eluent A(0,6% phosphoric acid) 1,51,8; eluent B acetonitrile. In order to separate nicotinic and ascorbic acid, stationary phases Luna C18(2) and Gemini C18, and to separate riboflavin and riboflavin-5¢- phosphate correspondently stationary phases Synergi Hydro RP and Zorbax SB-C18 are more preferable. The suggested technique was applied for the determination of vitamins in commercial samples of multivitamin pharmaceuticals and premixes. The obtained results were in good conclusion with passport data.
Moscow University Chemistry Bulletin.
2010, Vol. 51, No. 4, P. 315

Copyright (C) Chemistry Dept., Moscow State University, 2002
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