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V. A. Kudrinskaya, S. G. Dmitrienko, Yu. A. Zolotov

Spectrophotometric determination of flavonoids by the reaction with (4-nitrophenil)diazonium tetrafluoroborate


(4-Nitrophenil)diazonium tetrafluoroborate (4-NPD) has been suggested as a reagent for the spectrophotometric determination of flavonoids. It has been shown that in alkaline medium 4-NPD enters into the diazotization reaction with quercetin, naringenin, chrysin, morin, rutin and naringin to form peach-coloured products. The conditions for the spectrophotometric reaction have been optimized. Spectrophotometric method for the determination of flavonoids with detection limits (1.5 3.9)107M (0.05 0.16 mg/mL) has been developed. Quercetin has been determined in some pharmaceutical compositions.
Moscow University Chemistry Bulletin.
2010, Vol. 51, No. 4, P. 296

Copyright (C) Chemistry Dept., Moscow State University, 2002
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