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N. L. Klyachko, S. A. Legotsky, P. A. Levashov, V. M. Popova, A. Timasheva, N. G. Belogurova, I. A. Dyatlov, A. V. Levashov
Endolysin
of bacteriophage SPZ7: the effect of “helpers” on the enzyme lytic activity
towards gram-negative microorganisms
Abstract
Bacteriophage SPZ7 endolysin functioning in the lysis of gram-negative
bacteria from “outside” was studied. Significant (1.5–3 times) increase of
endolysin activity towards S. enteritidis N60 and E. coli TG1 was
shown in the presence of high molecular surfactants, pluronics, with large
hydrophobic block; hen egg lysozyme, and peptide antibiotics, colistin in low
concentrations. An approach developed can be an important for increasing an
efficiency of pharmaceutical antibacterial preparations based on bacteriophage
enzymes working against gram-negative microorganisms.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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