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M. M. Ulyasheva, M. Yu. Rubtzova, T. Bachmann, A. M. Yegorov

Optimization of hybridization analysis on DNA-microarrays with colorimetric detection on basis of horseradish peroxidase

Abstract

The method of hybridization analysis on DNA-microarrays with colorimetric detection on basis of horseradish peroxidase was developed. The effectiveness of biotin incorporation in nucleotide sequence during PCR and the influence of different hybridization temperatures, molarity of hybridization buffer and lengths of labeled DNA fragments on sensitivity and specificity of hybridization signals were analyzed. The method developed was applied for genotyping of CTX-M І-lactamases.
Moscow University Chemistry Bulletin.
2008, Vol. 49, No. 2, P. 96
   

Copyright (C) Chemistry Dept., Moscow State University, 2002
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