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G. V. Presnova, M. Yu. Rubtzova, V. V. Shumyantzeva, T. V. Bulko, A. M. Yegorov

Comparative immobilization of antibodies on a surface of modified screen-printed graphite electrodes

Abstract

The study of immobilization of polyclonal antibodies on a surface of screen-printed graphite electrodes (SPE) was carried out. The methods examined involved physical adsorbtion and the immobilization in the films of DDAB and films of DDAB and gold nanoparticles. The maximal electrochemical response was registered on the SPE modified by films of DDAB and gold nanoparticles. We have found that such films change markedly the surface morphology and the electrochemical signal is more intensive and stable. In this case the concentration of the immobilized antibodies increases, their activity remains. The detection limit for enzyme label (horseradish peroxidase) was 0.02 ng/ml of probe.
Moscow University Chemistry Bulletin.
2008, Vol. 49, No. 2, P. 91
   

Copyright (C) Chemistry Dept., Moscow State University, 2002
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