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A. V. Ivanov, D. V. Kurek, M. S. Vakstein, A. P. Bayunov
The
ionic strength gradient during the chromatofocusing within the carboxylic
columns
Abstract
The strong electrolyte presence in the mobile phases
influences essentially upon pH gradient profile in the chromatofocusing. The
current change of the strong electrolyte concentration in the system for
chromatofocusing was investigated. It was shown that the ionic strength
gradient gets out more earlier than pH gradient; the “anomalous” segments on
the pH gradient profile clash with the general change of ionic strength in the
system. The pH gradients, formed under the identical conditions on the
carboxylic sorbents MacroPrep 50 CM and MN were compared. The influence of KNO3 concentration in
both mobile phases on the pH gradient profiles was studied. The optimal systems
for formation of the smoother, linear pH gradients were selected.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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