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A. D. Ilyinб, E. P. Segura-Ceniceros, M. G. Hernбndez-Vazquez, R. Chбvez-Chбvez, Ye. Bogachiova, L. E. Elizalde-Herrera

Improvement of PGH-synthase stability in pectin films used in healing of skin wounds

Abstract

Prostaglandin H synthase (PGHS, E.C. 1.14.99.1) and prostaglandins mediate a variety of physiological and pathological processes. Herein, we report a new method of PGHS immobilization applicable to the treatment of skin injuries, using natural polymer pectin as a support matrix. PGHS was isolated from bovine vesicular glands, immobilized on pectin, and tested for stability and activity. The enzyme retained up to 88% of its activity at –15°C during first 5 days and 41% after 60 days of storage. The effectiveness of immobilized PGHS on tissue repair was tested in CD1 mice by measuring linear size of PGHS treated and untreated wound. Application of PGHS accelerated wound healing and stimulated prostaglandin E2 (PGE2) generation in the wound. Immobilized PGHS also increased specific rate of healing progression (m*) that was calculated as the tangent of the inclination angle of lines obtained in semi-logarithmic coordinates. A kinetic scheme was proposed to describe the process of wound healing as the function of PGHS concentration.
Moscow University Chemistry Bulletin.
2006, Vol. 47, No. 2, P. 122
   

Copyright (C) Chemistry Dept., Moscow State University, 2002
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