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E. G. Sadykhov, A. E. Serov, I. E. Yasny, N. S. Voinova, A. A. Alekseeva, A. S. Petrov, V. I. Tishkov

NAD+-Dependent Formate dehydrogenase from Arabidopsis thaliana and soya: expression in E. coli cells and kinetic properties of recombinant enzymes


It is the first case of successful expression in E.coli cells of NAD+-dependent formate dehydrogenases (EC from plants Arabidopsis thaliana and soya Gyicine max as active and soluble enzymes. Both enzymes were obtained in homogeneous form and kinetic properties of recombinant enzymes were studied. It was shown that new formate dehydrogenases have lower Km values for coenzyme NAD+ and second substrate formate compared to ones for the enzymes from bacteria and yeasts. Comparison of kinetic properties of plant formate dehydrogenases with NAD+ and NADP+ has shown that its coenzyme specificity is similar to one for the from bacterium from Pseudomonas sp.101.
Moscow University Chemistry Bulletin.
2006, Vol. 47, No. 1, P. 31

Copyright (C) Chemistry Dept., Moscow State University, 2002
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