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D. A. Gudkov, Yu. A. Votchitseva, E. N. Efremenko, S. D. Varfolomeyev

Paraoxon Hydrolysis catalyzed by organophosphate hydrolase containing the polyhistidine tag at the C-terminus of protein molecule


The non- Michaelis kinetics of paraoxon hydrolysis catalyzed by the organophosphate hydrolase, containing the hexahistidine tag at the C-terminus, was revealed. The appearance of catalytically active metal-complex formed by the hexahistidine tag, introduced into the genetically modified OPH structure, and the Co2+ ions, added to the reaction medium to activate the OPH being metalloenzyme, additionally to the native active site was supposed.
Moscow University Chemistry Bulletin.
2006, Vol. 47, No. 1, P. 15

Copyright (C) Chemistry Dept., Moscow State University, 2002
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