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I. S. Alpeeva, M. M. Anokhina, V. N. Tashlitsky, A. M. Kopylov

Determination of the part of transcription repressor protein TetR(D) active to tetra cycline-binding by competitive binding method

Abstract

The competitive binding isotherms of tetracycline and radioactive 7-[3H]-tetracycline with transcription represser protein TetR(D) were obtained. Mathematical model of the competitive binding which enables to calculate the part of TetR(D) active to tetracycline-binding were proposed. The application of this correction for calculation of apparent association constants allows the data obtained for different protein preparation to be unified. This method can be applied for calculation of apparent association constants for various protein complexes.
Moscow University Chemistry Bulletin.
2005, Vol. 46, No. 4, P. 263
   

Copyright (C) Chemistry Dept., Moscow State University, 2002
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