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O. N. Koroleva, V. L. Drutsa
An investigation
of transcription of the dna matrices
containing pyrophosphate internucleotide moieties
Abstract
DNA fragments, containing pyrophosphate
internucleotide groups, were prepared by chemical-enzymatic approach. The
effects of this modification on transcription with RNA polymerase of
Escherichia coli were studied. By in vitro transcription experiments
it has been shown, that RNA polymerase could bypass pyrophosphate group,
although the transcription of modified DNA fragments was less efficient then
that of natural ones, indicating a possibility of a brief pausing of the enzyme
at the modification site.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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