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O. N. Koroleva, V. L. Drutsa

An investigation of transcription of the dna matrices containing pyrophosphate internucleotide moieties


DNA fragments, containing pyrophosphate internucleotide groups, were prepared by chemical-enzymatic approach. The effects of this modification on transcription with RNA polymerase of Escherichia coli were studied. By in vitro transcription experiments it has been shown, that RNA polymerase could bypass pyrophosphate group, although the transcription of modified DNA fragments was less efficient then that of natural ones, indicating a possibility of a brief pausing of the enzyme at the modification site.
Moscow University Chemistry Bulletin.
2003, Vol. 44, No. 3, P. 203

Copyright (C) Chemistry Dept., Moscow State University, 2002
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