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Ilyina A., Castillo Sánchez M. I., Villarreal Sánchez J. A., Ramírez Esquivel G., Candelas Ramírez J.
Isolation of soil bacteria for bioremediation
of hydrocarbon contamination
Abstract
Bacterial strains were isolated from sites
impacted by spill of petroleum hydrocarbons for the development a product
(COBE-10) applicable in soil bioremediation. Initially, 82 bacterial strains
were isolated in selective agars (agar diesel, combustion oil and petroleum).
After monitoring absorbance change of mineral media (containing petroleum as
sole carbon source) inoculated by isolated strains, 30 strains were selected.
The strains were evaluated for their potential to degrade the hydrocarbons of
petroleum in soil, artificially contaminated under laboratory conditions. Based
on DTPH/day, finally 6 strains were selected. Seven carrier materials were
tested to select suitable vehicle for final formulation of COBE-10. Ex -situ evaluation of the developed
product (COBE-10) was performed in field with soils contaminated with diesel
and refinery wastes. The field test showed a high efficiency of biopreparation
COBE-10.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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