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Mukhitova F., Kashirina E.
Biocatalytic transformation of benzaldehyde
Abstract
The aim of the present investigation was to
study the possibilities of cell-free extracts of D.desulfuricans B-1388 to catalyze the reduction of aromatic
aldehyde - benzaldehyde to benzyl
alcohol. Reaction was carried out in anaerobic conditions in tris-HCl buffer.
It was shown that in the presence of molecular hydrogen and cell-free extracts
of D.desulfovibrio benzaldehyde is
transformed into corresponding benzyl alcohol and benzoic acid. When the
hydrogen is changed by inert gas - argon, only benzoic acid is observed in
reaction medium.
The influence of physico-chemical conditions on the product yield was
investigated. It was found that maximum production of benzyl alcohol take place
at 100% H2 in gaseous phase. It was found that optimal meanings are
of 7.0 for pH and 250C for
temperature. The highest alcohol production was observed at the lowermost
ionic strength.
In all experiments there was bidirectional
transformation of benzaldehyde so both
hydrogenase and aldehyde dehydrogenase catalyze this process.
Copyright (C) Chemistry Dept., Moscow State University, 2002
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